rhaB [2019-07-09 11:27:50]

rhamnulokinase

locus: BSU31200

pI: 5.42

mw: 54.57 kDa

protein length: 485 aa

gene length: 1458 bp

function: utilization of rhamnose

product: rhamnulokinase

essential: no

ec: 2.7.1.5

synonyms: yulC

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.12|Utilization of rhamnose]

Gene

Coordinates: 3,199,565 3,201,022

The protein

Catalyzed reaction/ biological activity

• ATP L-rhamnulose = ADP L-rhamnulose 1-phosphate [Pubmed|26712933]

Protein family

• rhamnulokinase family (according to Swiss-Prot)

Structure

• [PDB|2UYT] (the enzyme of ''E. coli'')

Expression and Regulation

Operons

• genes: [gene|C32AE30BF7931D6171E9DE75C67504F81DD0F227|rhaEW]-[gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]
  description: [Pubmed|26712933,22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|26712933], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|F574142C1E61788FF8F4B61280AD4F87292D08DF|RhaR]: repression, [Pubmed|26712933], in [regulon|F574142C1E61788FF8F4B61280AD4F87292D08DF|RhaR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|26712933], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]

• genes: [gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]
  description: [Pubmed|22383849]

  regulation

  • expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]

Biological materials

Mutant

• MGNA-B545 (yulC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1544 NBRP B. subtilis, Japan]
• BKE31200 ([gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAATGGCAGTATAAATCA, downstream forward: _UP4_AAATGATGAAGAGGTGAAAA
• BKK31200 ([gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCAATGGCAGTATAAATCA, downstream forward: _UP4_AAATGATGAAGAGGTGAAAA

References

• 26712933,16674975,24391637,22383849